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dy3144 05  (R&D Systems)


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    Structured Review

    R&D Systems dy3144 05
    Dy3144 05, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+je+mcp/Rat+JE%2FMCP-1%2FCCL2+DuoSet+ELISA/pm41833640-172-24-29
    Average 94 stars, based on 21 article reviews
    dy3144 05 - by Bioz Stars, 2026-08
    94/100 stars

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    (A) Daily and (B) cumulative NO release from IVD explants with and without TNFα in macrophage media ( n = 3). Cumulative (C) TNFα, (D) IL‐6, (E) IL‐13, and (F) <t>CCL2</t> in media supernatants of IVD explants with and without TNFα stimulation ( n = 3; Black * p < 0.05 IVD significantly different from IVD + TNFα within timepoint, colored * p < 0.05 Group of corresponding color significantly different between timepoints).
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    Image Search Results


    (A) Daily and (B) cumulative NO release from IVD explants with and without TNFα in macrophage media ( n = 3). Cumulative (C) TNFα, (D) IL‐6, (E) IL‐13, and (F) CCL2 in media supernatants of IVD explants with and without TNFα stimulation ( n = 3; Black * p < 0.05 IVD significantly different from IVD + TNFα within timepoint, colored * p < 0.05 Group of corresponding color significantly different between timepoints).

    Journal: JOR Spine

    Article Title: Macrophage Subtypes Distinctly Impact Intervertebral Disc Inflammation and Extracellular Matrix Composition

    doi: 10.1002/jsp2.70143

    Figure Lengend Snippet: (A) Daily and (B) cumulative NO release from IVD explants with and without TNFα in macrophage media ( n = 3). Cumulative (C) TNFα, (D) IL‐6, (E) IL‐13, and (F) CCL2 in media supernatants of IVD explants with and without TNFα stimulation ( n = 3; Black * p < 0.05 IVD significantly different from IVD + TNFα within timepoint, colored * p < 0.05 Group of corresponding color significantly different between timepoints).

    Article Snippet: Production of inflammatory cytokines and chemokines was also evaluated using the Rat CCL2 DuoSet ELISA (R&D Systems, Cat#. DY3144‐05) and the LEGENDPlex Rat Th Cytokine Panel V02 (BioLegend, Cat#. 741220) multiplex assay containing 13 analytes: TNFα, IL‐6, IL‐13, IFNγ, IL‐17A, IL‐17F, IL‐5, IL‐2, IL‐4, IL‐10, IL‐22, GM‐CSF, and IL‐9.

    Techniques:

    (A) Daily and (B) cumulative NO release from TNFαα‐stimulated IVD explants alone and co‐cultured with M1 macrophages (IVD + TNFα: n = 3, IVD + TNFα + M1: n = 6). Cumulative concentrations of (C) TNFα, (D) IL‐6, (E) IL‐13, and (F) CCL2 in media supernatants from M1 macrophage‐IVD explant co‐culture in TNFα ( n = 3). (G) Daily and (H) cumulative NO release from TNFα‐stimulated IVD explants alone and co‐cultured with M2 macrophages (IVD + TNFα: n = 3, IVD + TNFα + M2: n = 6). Cumulative concentrations of (I) TNFα, (J) IL‐6, (K) IL‐13, and (L) CCL2 in media supernatants from M2 macrophage‐IVD explant co‐culture in TNFα ( n = 3; Black * p < 0.05 IVD + TNFα significantly different from IVD + TNFα + M1/M2 within timepoint, colored * p < 0.05 Group of corresponding color significantly different between timepoints, ^ p < 0.05 IVD + TNFα + M1/M2 signficantly different from TNFα + M1/M2, + p < 0.05 IVD + TNFα significantly different from TNFα + M1/M2).

    Journal: JOR Spine

    Article Title: Macrophage Subtypes Distinctly Impact Intervertebral Disc Inflammation and Extracellular Matrix Composition

    doi: 10.1002/jsp2.70143

    Figure Lengend Snippet: (A) Daily and (B) cumulative NO release from TNFαα‐stimulated IVD explants alone and co‐cultured with M1 macrophages (IVD + TNFα: n = 3, IVD + TNFα + M1: n = 6). Cumulative concentrations of (C) TNFα, (D) IL‐6, (E) IL‐13, and (F) CCL2 in media supernatants from M1 macrophage‐IVD explant co‐culture in TNFα ( n = 3). (G) Daily and (H) cumulative NO release from TNFα‐stimulated IVD explants alone and co‐cultured with M2 macrophages (IVD + TNFα: n = 3, IVD + TNFα + M2: n = 6). Cumulative concentrations of (I) TNFα, (J) IL‐6, (K) IL‐13, and (L) CCL2 in media supernatants from M2 macrophage‐IVD explant co‐culture in TNFα ( n = 3; Black * p < 0.05 IVD + TNFα significantly different from IVD + TNFα + M1/M2 within timepoint, colored * p < 0.05 Group of corresponding color significantly different between timepoints, ^ p < 0.05 IVD + TNFα + M1/M2 signficantly different from TNFα + M1/M2, + p < 0.05 IVD + TNFα significantly different from TNFα + M1/M2).

    Article Snippet: Production of inflammatory cytokines and chemokines was also evaluated using the Rat CCL2 DuoSet ELISA (R&D Systems, Cat#. DY3144‐05) and the LEGENDPlex Rat Th Cytokine Panel V02 (BioLegend, Cat#. 741220) multiplex assay containing 13 analytes: TNFα, IL‐6, IL‐13, IFNγ, IL‐17A, IL‐17F, IL‐5, IL‐2, IL‐4, IL‐10, IL‐22, GM‐CSF, and IL‐9.

    Techniques: Cell Culture, Co-Culture Assay